The sequences of your ColE1 origin primers Employed in two-fragment cloning had been GGAGCGAACGACCTACACCGAACTGAGATACCTACAGCG and CGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCC. All primers were purchased as desalted oligonucleotides and applied with out even further purification. Verify primer sequences to guarantee they are complementary to the specified focus on to the template DNA strand.... https://mutagenesis97531.atualblog.com/27696445/not-known-details-about-site-directed-mutagenesis